Biotechnology

Recombinant DNA technology is a 3 credit core course for the M.Sc. Semester III Biotechnology program.  This is an advanced course dealing with the tools and techniques involved in manipulating DNA. The various modules elaborate the different enzymes, the types of vectors used, the expression systems, the heterologous host systems used as well as the various cloning strategies and the processes involved therein.  In addition techniques such as PCR, blotting, site directed mutagenesis, gene transfer and various screening strategies are  included. 

After completing the course the student will be able to

• Elaborate the different enzymes, vectors, as well as cloning strategies 

• Apply the different enzymes used in rDNA technology 

• Use different types of vectors for cloning 

• Produce a genomic DNA library and screening for recombinants 

• Construct a probe and do blotting techniques 

• Apply site directed mutagenesis technique 

•  Employ different types of PCR techniques for gene amplification and clone the amplicon 

• Demonstrate heterologous gene expression 

• Compare various genome editing tools